NOVAMATRIX-3D™ applications

NOVAMATRIX-3D™ applications

NOVAMATRIX-3D™ is a 3D cell culture system mimicking the configuration of living organisms. NOVAMATRIX-3D™ alginate foams can be used for research purposes in fields such as:

  • Preclinical drug testing
  • Tumors and organoids for cancer research
  • Tissue engineering
  • Stem cell research

An in-vivo mimicking environment

Figure 1 – micrographs of alginate-based foam scaffolds, where pore sizes were observed to be ~ 100 to 700 µm in diameter

NOVAMATRIX-3D™ is an effective system to seed, grow and harvest various cell types. The alginate-based foams scaffolds are engineered to have a broad range of pore sizes, e.g., ranging from ~100 to 700 µm in diameter. This ensures an effective encapsulation when the pores are hydrated with a suspension of cells in alginate solution. Each disc is capable of absorbing approximately 20-30x of its own weight of buffer or medium.

Figure 2 – Scheme depicting cell attachment procedure to hydrate foam through in situ alginate gelation.

Hydrated NOVAMATRIX-3D™ alginate foam discs exhibit a Young’s modulus of approximately 1-3 kPa under the recommended seeding protocol of a 0.5 wt% alginate solution, which falls within the comparative range of soft tissue stiffness. As such, these alginate-based scaffolds can mimic an in-vivo environment, promoting the complex interactions between cells and the extracellular matrix.

Cell growth in NOVAMATRIX-3D™

Figure 3 – Growth of NIH3T3, HT-29 and hMSC cells in NOVAMATRIX-3D™ over two weeks. Scale 100 μm.

NIH3T3, HT29 and hMSC cells showed high viability in NOVAMATRIX-3D™ after cell seeding and encapsulation using NOVATACH™ peptide-coupled alginate. The cells showed excellent metabolic activity and 85-95% viability, demonstration cell health during encapsulation and dissolution.

Figure 4 – Cell viability (%) for various cell types encapsulated in NOVAMATRIX-3D™ and recovered using dissolution buffer. Viability was assessed with Trypan blue.

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